,MGHK,

acyl-(acyl carrier protein) thioesterase (FatB)

LOCUS T88656 343 bp mRNA EST 04-AUG-1998

DEFINITION 12352 Lambda-PRL2 Arabidopsis thaliana cDNA clone 161A11T7, mRNA

sequence.

ACCESSION T88656

NID g2763922

KEYWORDS EST.

SOURCE thale cress.

ORGANISM Arabidopsis thaliana

Eukaryota; Viridiplantae; Charophyta/Embryophyta group;

Embryophyta; Tracheophyta; euphyllophytes; Spermatophyta;

Magnoliophyta; eudicotyledons; Rosidae; Capparales; Brassicaceae;

Arabidopsis.

REFERENCE 1 (bases 1 to 343)

AUTHORS Newman,T., deBruijn,F.J., Green,P., Keegstra,K., Kende,H.,

McIntosh,L., Ohlrogge,J., Raikhel,N., Somerville,S., Thomashow,M.,

Retzel,E. and Somerville,C.

TITLE Genes galore: a summary of methods for accessing results from

large-scale partial sequencing of anonymous Arabidopsis cDNA clones

JOURNAL Plant Physiol. 106, 1241-1255 (1994)

MEDLINE 95148729

COMMENT

Contact: Thomas Newman

MSU-DOE Plant Research Laboratory

Michigan State University

MSU-DOE-PRL, Michigan State University,Plant Biology Bldg.,E.

Lansing,Mi

Tel: 517-353-0854

Fax: 517-353-9168

Email: 22313tcn@ibm.cl.msu.edu

Seq primer: T7 dye primer.

FEATURES Location/Qualifiers

source 1. .343

/organism="Arabidopsis thaliana"

/strain="var columbia"

/note="Vector: lambda Zip-Lox; Site_1: Sal; Site_2: Not;

Lambda PRL2 is a cDNA library derived from equal

quantities of 4 pools of mRNA. The mRNA sources were 1) 7

day germinated etiolated seedlings; 2) tissue culture

grown roots; 3) staged plants half with 24 hour light

cycle, half on 16 hr light, 8 hour dark- rosettes; 4)

same plants as 3 but aerial tissue (stems, flowers and

siliques. The vector is BRL's lambda Zip-Lox. The cDNA

inserts were directionally cloned with Sal-Not arms using

oligo dT primed cDNA."

/db_xref="taxon:3702"

/clone="161A11T7"

/clone_lib="Lambda-PRL2"

BASE COUNT 89 a 46 c 95 g 100 t 13 others

ORIGIN

T88656 Length: 343 October 26, 1998 16:17 Type: N Check: 6716 ..

1 GGCGCTTAAT CATGTTAAGA CTGCTGGATT GCTTGGAGAT GGGTTTGGCT

51 CTACACCTGA GATGTTTAAG AAGAACTTGA TATGGGTTGT CACTCGTATG

101 CAGGTTGTGG TTGATAAATA TCCTACTTGG GGAGATGTTG TTGAAGTAGA

151 CACCTNGGTC AGTCAGTCTG GAAAGAATGG TATGCGTCGT GATTGGCTAG

201 TNCGGGACTG TAATACTGGA GAAACCTTAA CACGAGCATC AAGTGTGTGG

251 GTGATGATGA ATAANCTNAC ANGGAGATTT TCAAAGATTC CTTAANGAAG

301 GTTCGNGNGG GAAATAGAGC CTTANTTTTN TGNAATNCNT TGA

BLASTX 10-27-98: acyl-(acyl carrier protein) thioesterase (FatB), identification in the first 2 hits is wrong

Score E

Sequences producing significant alignments: (bits) Value

pir||S51699 oleoyl-[acyl-carrier-protein] hydrolase (EC 3.1.2.1... 199 7e-51

pir||S51697 oleoyl-[acyl-carrier-protein] hydrolase (EC 3.1.2.1... 199 7e-51

gi|1930081 (U92878) acyl-ACP thioesterase [Garcinia mangostana] 176 3e-44

pir||S69196 acyl-(acyl carrier protein) thioesterase (clone TE ... 175 1e-43

gi|495227 (X76561) acyl-ACP thioesterase [unidentified] 168 2e-41

gi|2687425 (AF036565) FatB thioesterase [Helianthus annuus] 166 5e-41

gi|758701 (U17076) 16:0-ACP thioesterase preprotein [Cuphea hoo... 164 1e-40

gi|2459531 (U65643) acyl-ACP thioesterase [Myristica fragrans] 160 3e-39

gi|2459529 (U65642) acyl-ACP thioesterase [Myristica fragrans] 160 3e-39

gi|1215720 (U38189) thioesterase [Cuphea palustris] >gi|1588564... 148 9e-36

gi|1336006 (U56103) acyl-ACP thioesterase [Cuphea wrightii] 146 3e-35

gi|1336008 (U56104) acyl-ACP thioesterase [Cuphea wrightii] 144 2e-34

gi|2459533 (U65644) acyl-ACP thioesterase [Ulmus americana] 143 3e-34

gi|1215718 (U38188) thioesterase [Cuphea palustris] >gi|1588563... 141 2e-33

gi|1143156 (U31813) acyl-ACP thioesterase [Cinnamomum camphora]... 131 1e-30

pir||A40229 laurate acyl-carrier protein thioesterase - Califor... 131 2e-30

gi|1292906 (U39834) FatB2 [Cuphea hookeriana] 128 1e-29

gi|595955 (U17097) Uc FatB2 [Umbellularia californica] 123 4e-28

gi|595957 (U17098) Br FatA1 [Brassica rapa] 106 4e-23

gi|1930079 (U92877) acyl-ACP thioesterase [Garcinia mangostana] 104 3e-22

pir||S51698 oleoyl-[acyl-carrier-protein] hydrolase (EC 3.1.2.1... 103 5e-22

gi|511497 (L20978) oleoyl-acyl carrier protein thioesterase [Co... 102 1e-21

pir||S40407 oleoyl-[acyl-carrier-protein] hydrolase (EC 3.1.2.1... 101 2e-21

pir||S40408 oleoyl-[acyl-carrier-protein] hydrolase (EC 3.1.2.1... 101 2e-21

gi|404026 (M96568) oleoyl-acyl carrier protein thioesterase [Ca... 100 5e-21

gi|1930077 (U92876) acyl-ACP thioesterase [Garcinia mangostana] 100 5e-21

gi|404028 (M96569) oleoyl-acyl carrier protein thioesterase [Ca... 99 7e-21

pir||S51699 oleoyl-[acyl-carrier-protein] hydrolase (EC 3.1.2.14) - Arabidopsis

thaliana >gi|804948 (Z36911) acyl-(acyl carrier protein)

thioesterase [Arabidopsis thaliana]

Length = 412

Score = 199 bits (500), Expect = 7e-51

Identities = 98/109 (89%), Positives = 98/109 (89%)

Query: 2 ALNHVKTAGLLGDGFGSTPEMFKKNLIWVVTRMQVVVDKYPTWGDVVEVDTXVSQSGKNG 181

ALNHVKTAGLLGDGFGSTPEMFKKNLIWVVTRMQVVVDKYPTWGDVVEVDT VSQSGKNG

Sbjct: 169 ALNHVKTAGLLGDGFGSTPEMFKKNLIWVVTRMQVVVDKYPTWGDVVEVDTWVSQSGKNG 228

Query: 182 MRRDWLVRDCNTGETLTRASSVWVMMNXLTXRFSKIP*XRFXXEIEPXF 328

MRRDWLVRDCNTGETLTRASSVWVMMN LT R SKIP EIEP F

Sbjct: 229 MRRDWLVRDCNTGETLTRASSVWVMMNKLTRRLSKIP-EEVRGEIEPYF 276

pir||S51697 oleoyl-[acyl-carrier-protein] hydrolase (EC 3.1.2.14) - Arabidopsis

thaliana >gi|2129530|pir||S69195 acyl-(acyl carrier

protein) thioesterase (clone TE 1-1) - Arabidopsis

thaliana >gi|634003 (Z36910) acyl-(acyl carrier protein)

thioesterase [Arabidopsis thaliana]

Length = 412

Score = 199 bits (500), Expect = 7e-51

Identities = 98/109 (89%), Positives = 98/109 (89%)

Query: 2 ALNHVKTAGLLGDGFGSTPEMFKKNLIWVVTRMQVVVDKYPTWGDVVEVDTXVSQSGKNG 181

ALNHVKTAGLLGDGFGSTPEMFKKNLIWVVTRMQVVVDKYPTWGDVVEVDT VSQSGKNG

Sbjct: 169 ALNHVKTAGLLGDGFGSTPEMFKKNLIWVVTRMQVVVDKYPTWGDVVEVDTWVSQSGKNG 228

Query: 182 MRRDWLVRDCNTGETLTRASSVWVMMNXLTXRFSKIP*XRFXXEIEPXF 328

MRRDWLVRDCNTGETLTRASSVWVMMN LT R SKIP EIEP F

Sbjct: 229 MRRDWLVRDCNTGETLTRASSVWVMMNKLTRRLSKIP-EEVRGEIEPYF 276

gi|1930081 (U92878) acyl-ACP thioesterase [Garcinia mangostana]

Length = 406

Score = 176 bits (443), Expect = 3e-44

Identities = 84/97 (86%), Positives = 89/97 (91%)

Query: 2 ALNHVKTAGLLGDGFGSTPEMFKKNLIWVVTRMQVVVDKYPTWGDVVEVDTXVSQSGKNG 181

ALNHVKTAGLLGDGFGSTPEM K+NLIWVVT+MQV VD+YPTWGDVV+VDT VS SGKNG

Sbjct: 157 ALNHVKTAGLLGDGFGSTPEMSKRNLIWVVTKMQVEVDRYPTWGDVVQVDTWVSASGKNG 216

Query: 182 MRRDWLVRDCNTGETLTRASSVWVMMNXLTXRFSKIP 292

MRRDWL+RD NTGETLTRASSVWVMMN LT R SKIP

Sbjct: 217 MRRDWLLRDGNTGETLTRASSVWVMMNKLTRRLSKIP 253

pir||S69196 acyl-(acyl carrier protein) thioesterase (clone TE 3-2) -

Arabidopsis thaliana

Length = 412

Score = 175 bits (438), Expect = 1e-43

Identities = 87/109 (79%), Positives = 89/109 (80%)

Query: 2 ALNHVKTAGLLGDGFGSTPEMFKKNLIWVVTRMQVVVDKYPTWGDVVEVDTXVSQSGKNG 181

ALNHVKTAGLLGDGFGSTPEMFKKNLIWVVTRMQVVVDKYPTWGDVVEVDT VSQSGKNG

Sbjct: 169 ALNHVKTAGLLGDGFGSTPEMFKKNLIWVVTRMQVVVDKYPTWGDVVEVDTWVSQSGKNG 228

Query: 182 MRRDWLVRDCNTGETLTRASSVWVMMNXLTXRFSKIP*XRFXXEIEPXF 328

MRRDWLVRDCNTG R++ WVMM T R KIP EIEP F

Sbjct: 229 MRRDWLVRDCNTGTLTGRSTVKWVMMKLDTRRSQKIP-EEVRGEIEPYF 276

gi|495227 (X76561) acyl-ACP thioesterase [unidentified]

Length = 414

Score = 168 bits (420), Expect = 2e-41

Identities = 79/109 (72%), Positives = 91/109 (83%)

Query: 2 ALNHVKTAGLLGDGFGSTPEMFKKNLIWVVTRMQVVVDKYPTWGDVVEVDTXVSQSGKNG 181

ALNHVK+AGLL DGFG TPEMFK++LIWVV +MQV+V++YPTWGD VEV+T V++SGKNG

Sbjct: 168 ALNHVKSAGLLNDGFGRTPEMFKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNG 227

Query: 182 MRRDWLVRDCNTGETLTRASSVWVMMNXLTXRFSKIP*XRFXXEIEPXF 328

MRRDWL+ DCNTGE LTRASSVWVMMN T + SKIP EIEP F

Sbjct: 228 MRRDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIP-DEVRHEIEPHF 275

gi|2687425 (AF036565) FatB thioesterase [Helianthus annuus]

Length = 430